CalCOFI Cruise 9602 & 9604 Introduction
INTRODUCTION
The data in this report were collected during cruises 9602* and 9604 of the
California Cooperative Oceanic Fisheries Investigations (CalCOFI) program aboard
the NOAA ship RV David Starr Jordan. The CalCOFI program was organized in the
late 1940's to study the causes of variations in population size of fishes of
importance to the State of California. It is carried out by NOAA's National
Marine Fisheries Service Southwest Fisheries Science Center, the California
Department of Fish and Game, and the Marine Life Research Group (MLRG) at
Scripps Institution of Oceanography (SIO). MLRG contributes to this program by
investigations of the physical, chemical and biological structure of the
California Current. Data from CalCOFI cruises 9602 and 9604 were collected and
processed by personnel of the Marine Life Research Group and the Southwest
Fisheries Science Center. Volunteers and other SIO staff members also assisted
in the collection of data and chemical analyses at sea.
STANDARD PROCEDURES
Rosette Cast Data
At each station on cruises 9602 and 9604 a Sea-Bird Electronics, Inc.,
Conductivity-Temperature-Depth (CTD) instrument was deployed with a 24-place
General Oceanics rosette. The rosette was equipped with 24 ten-liter plastic
(PVC) bottles. The CTD/rosette cast usually sampled 20 depths to a maximum
sampling depth of 525 meters, bottom depth permitting. Occasional stations have
multiple bottles tripped at the same depth to provide more water for ancillary
programs. Pressures and temperatures assigned to the water sample data were
derived from the CTD signals recorded just prior to the bottle trip. Pressures
have been converted to depths by the Saunders (1981) pressure-to-depth
conversion technique. CTD temperatures reported with the bottle data have been
rounded to the nearest hundredth of a degree Celsius. Salinity, oxygen and
nutrients were determined at sea for all depths sampled. Chlorophyll-a and
phaeopigments were determined at sea within the top 200 meters, bottom depth permitting.
Salinity samples were collected from all rosette bottles and analyzed at sea
using a Guildline model 8410 Portasal salinometer. The results were compared
with the CTD salinity in order to verify that the rosette bottle did not mis-
trip or leak. The salinometer was standardized before and after each group of
samples with substandard seawater. Periodic checks on the conductivity of the
substandard were made by comparison with IAPSO Standard Seawater batch P127.
Salinity values have been calculated from the algorithms for the Practical
Salinity Scale, 1978 (UNESCO, 1981a) and were reported to three decimal places,
provided that accepted standards were met. If only one determination per sample
was obtained, or there was doubt concerning the accuracy of the analytical
results, the salinities were reported to two decimal places. Dissolved
oxygen was determined by the Winkler method, as modified by Carpenter (1965),
using the equipment and procedure outlined by Anderson (1971). Percent oxygen
saturation was calculated from the equations of Weiss
(1970).
Silicate, phosphate, nitrate and nitrite nutrients were determined at sea
using an automated analyzer. The procedures used are similar to those described
in Atlas et al. (1971).
Samples for chlorophyll-a and phaeopigments were filtered onto Whatman GF/F
filters. The pigments were extracted with a cold extraction technique in 90%
acetone (Venrick and Hayward, 1984), and the fluorescence determined before and
after acidification with a Turner Designs fluorometer (Yentsch and Menzel, 1963;
Holm-Hansen et al. 1965).
Evaluation of the data involved comparisons with the CTD cast profiles,
adjacent stations and consideration of the variation of a property as a function
of density or depth and the relationships with other properties (Klein, 1973).
Estimates of precision of the standard techniques are given in SIO, 1991.
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* The first two digits represent the year and the last digits the month of the
cruise.
Primary Productivity Sampling
Primary productivity samples were taken each day shortly before local
apparent noon (LAN). Primary production was estimated from 14C uptake using a
simulated in situ technique. Light penetration was estimated from the Secchi
depth (assuming that the 1% light level is three times the Secchi depth). The
depths with ambient light intensities corresponding to light levels simulated by
the on-deck incubators were identified and sampled on the up rosette cast.
Occasionally an extra bottle or two were tripped in addition to the usual 20
levels sampled in the combined rosette- productivity cast in order to maintain
the normal sampling depth resolution. The ten-liter bottles were equipped with
epoxy-coated springs and Viton O-rings. Triplicate samples (two light and one
dark control) were drawn from each productivity sample depth into 250 ml
polycarbonate incubation bottles. Samples were inoculated with 10 æCi of 14C as
NaHCO3 (200 æl of 50 æCi/ml stock) prepared in a 0.3 g/liter solution of sodium
carbonate (Fitzwater et al. 1982). Samples were incubated from LAN to civil
twilight in seawater-cooled incubators with neutral-density screens which
simulate in situ light levels. At the end of the incubation, the samples were
filtered onto Millipore HA filters and placed in scintillation vials. One half
ml of 10% HCl was added to each sample. The sample was then allowed to sit,
without a cap, at room temperature for 12 hours (after Lean and Burnison, 1979).
Following this, 10 ml of scintillation fluor were added to each sample and the
samples were returned to SIO where the radioactivity was determined with a
scintillation counter. Salinity, oxygen, nutrients, chlorophyll-a and
phaeopigments were determined from all rosette productivity bottles.
Macrozooplankton Net Tows
Macrozooplankton was sampled with a 71 cm mouth diameter paired net (bongo
net) equipped with 0.505 mm plankton mesh. Bottom depth permitting, the nets
were towed obliquely from 210 meters to the surface. The tow time for a
standard tow was 21.5 minutes. Volumes filtered were determined from flowmeter
readings and the mouth area of the net. Only one sample of each pair was
retained and preserved. The biomass, as wet displacement volume, after removal
of large (>5 ml) organisms, was determined in the laboratory ashore. These
procedures are summarized in greater detail in Kramer et al. (1972).
Ancillary Programs
Several ancillary programs produced data on these cruises which are not
presented in this report. These programs include:
1) ADCP. Acoustic Doppler Current Profiler data were recorded continuously
along the ship's cruise track.
2) Avifauna Observation. Sea birds were counted within a 300 meter wide strip
off to one side of the ship. Counts were made while underway between stations
during periods of daylight. These counts were summed over 20 nautical mile (nm)
intervals, or the distance between consecutive stations, whichever was less.
3) Benthic sampling. Bottom samples were taken at two sites on cruise 9602 and
three sites on 9604. Samples were preserved for subsequent analysis of benthic
foraminifera, organic carbon analysis, and other faunal and geochemical
analyses.
4) Bio-optics. On 9602 and 9604 Bio-optical profiles were measured almost
daily using a variety of sensors, and spectral absorption by particulate and
soluble fractions was measured. On 9602 the bio-optics program also included
cyanobacteria microscopic counts by epifluorescence and phycoerythrin pigment
concentration determined by fluorescence spectroscopy.
5) Pigment studies. These included measurement of 14C incorporation into
pigments in incubated samples, phytoplankton pigment analyses of euphotic zone
samples using high performance liquid chromatography, phytoplankton fluorescence
measurements before and after DCMU addition, and nutrient enrichment experiments
to assess changes in phytoplankton populations as indicated by pigment
concentrations.
6) Underway Data. Continuous near surface measurements of temperature,
salinity and chlorophyll fluorescence were made from water pumped through the
ship, and the data were logged at one minute intervals. On 9604 sardine and
anchovy eggs were collected underway with a separate large volume pump. This
pump drew a continuous sample of approximately 640 liters per minute from which
eggs were concentrated and collected by a 505 um sieve system. Samples were
sequentially collected from this system periodically for enumeration of sardine
and anchovy eggs at sea and again ashore.
TABULATED DATA
Rosette Cast Data
The time reported is the Coordinated Universal Time (UTC) of the first
rosette bottle trip on the up cast. The rosette bottles tripped on the up cast
are reported as cast 2, where cast 1 is considered to be the down CTD cast. The
sample number reported is the cast number followed by a two digit rosette bottle
number. Bottom depths, determined acoustically, have been corrected using
British Admiralty Tables (Carter, 1980) and are reported in meters. Weather
conditions have been coded using WMO code 4501. Secchi depths and Forel water
color scales are also reported for most daylight stations.
Observed data from individual CTD/rosette trip levels are interpolated and
reported for standard depths. Interpolated or extrapolated standard level data
are noted by the footnote "ISL" printed after the depth. Multiple bottles
tripped at the same depth to provide water for ancillary programs are not used
in the calculation of standard depth data. Density-related parameters have been
calculated from the International Equation of State of Seawater 1980 (UNESCO,
1981, b). Computed values of potential temperature, sigma-theta, specific
volume anomaly (SVA), and dynamic height or geopotential anomaly are included
with both observed and interpolated standard depth levels.
On stations where primary productivity samples were drawn from six of the
rosette bottles, a footnote appears after each productivity depth sampled. The
corresponding primary productivity data are reported in a separate section
following the tabulated rosette cast data.
Primary Productivity Data
In addition to the normal hydrographic data also reported in the rosette
cast data section, the tabulated data include: the in situ light levels at which
the samples were collected, the uptake from each of the replicate light bottles,
uptake 1 and uptake 2, (which have been corrected for dark uptake by subtracting
the dark value), the mean of the two uptake values and the dark uptake. The
uptake values are totals for the incubation period. Also shown are the times of
LAN, civil twilight, and the value of the mean uptake integrated from the
surface to the deepest sample, assuming the shallowest value continues to the
surface and that negative values (when dark uptake exceeds light uptake) are
zero. The uptake data have been presented to two significant digits (values
<1.00) or one decimal (values >1.00). Precision of the higher production values
may not warrant all of the digits presented. Incubation time, LAN, and civil
twilight are given in local Pacific Standard Time (PST); to convert to UTC, add
eight hours to the PST time. Incubation light intensities are listed in a
footnote at the bottom of each page.
Macrozooplankton Data
Macrozooplankton biomass volumes are tabulated as total biomass volume
(cm3/1000m3 strained) and as the total volume minus the volume of larger
organisms under the heading "Small." Tow times are given in local PST (+8)
time.
FOOtnOTES
In addition to footnotes, special notations are used without footnotes because
the meaning is always the same.
D: CTD salinity value listed in place of normal ship-board salinity
analysis. ISL: After a depth value indicates that this is an interpolated or
extrapolated standard level.
U: Uncertain value. Values which are not used in interpolation
because they seem to be in error without apparent reason.