INTRODUCTION

The data presented in this report were collected during the 0304* cruise of the California Cooperative Oceanic Fisheries Investigations (CalCOFI) program aboard the aboard the RV Roger Revelle of Scripps Institution of Oceanography, University of California, San Diego. The CalCOFI program was organized in the late 1940’s to study the causes of variations in population size of fishes of importance to the State of California. It is carried out by NOAA’s National Marine Fisheries Service Southwest Fisheries Science Center, the California Department of Fish and Game, and the Integrative Oceanography Division (IOD) at Scripps Institution of Oceanography (SIO). IOD contributes to this program by investigations of the physical, chemical and biological structure of the California Current. Data from the cruises were collected and processed by personnel of the Integrative Oceanography Division and the Southwest Fisheries Science Center. Other SIO staff members and volunteers also assisted in the collection of data and chemical analyses at sea. CalCOFI data presented in this report and collected on previous cruises can be accessed at http://www.calcofi.org.

STANDARD PROCEDURES

CTD/Rosette Cast Data

A Sea-Bird Electronics, Inc., Conductivity-Temperature-Depth (CTD) instrument (Seabird 911, Serial number 1049) with a rosette was deployed at each station on these cruises. The rosette was equipped with 24 ten-liter plastic (PVC) bottles equipped with epoxy-coated springs and Viton O-rings. Each CTD/rosette cast usually sampled 20 depths to a maximum sampling depth of 525 meters, bottom depth permitting. Occasional stations have multiple bottles tripped at the same depth to provide more water for ancillary programs. The sample spacing was designed to sample depth intervals as close as 10 meters around the sharp upper thermocline features such as the chlorophyll, oxygen, nitrite maxima and the shallow salinity minimum. Salinity, oxygen and nutrients were determined at sea for all depths sampled. Chlorophyll-a and phaeopigments were determined at sea on samples from the top 200 meters, bottom depth permitting.

Pressures and temperatures assigned to the water sample data were derived from the CTD signals recorded just prior to the bottle trip. Pressures have been converted to depths by the Saunders (1981) pressure-to-depth conversion technique. CTD temperatures reported with the bottle data have been rounded to the nearest hundredth of a degree Celsius.

Salinity samples were collected from all rosette bottles and analyzed at sea using a Guildline model 8410 Portasal salinometer. Salinity samples were drawn into 200 ml Kimax high-alumina borosilicate bottles that were rinsed three times with sample prior to filling. The results were compared with the CTD salinity to verify that the rosette bottle did not mis-trip or leak. The salinometer was standardized before and after each group of samples with standardized seawater. Periodic checks on the conductivity of the standardized seawater were made by comparison with IAPSO Standard Seawater batch P140. Salinity values were calculated using the algorithms for the Practical Salinity Scale, 1978 (UNESCO, 1981a) and are reported to three decimal places, provided that accepted standards were met.

Dissolved oxygen samples were collected in calibrated 100 ml iodine flasks, allowing at least 200% overflow. The dissolved oxygen samples were analyzed at sea by the Winkler method, as modified by Carpenter (1965), using the equipment and procedure outlined by Anderson (1971). Percent oxygen saturation was calculated from the equations of Weiss (1970).

Nutrient samples were analyzed at sea by the Scripps Ocean Data Facility for dissolved silicate, phosphate, nitrate and nitrite using procedures similar to those described in Gordon et al., 1993. Samples were collected in 45 ml high-density polypropylene screw-capped tubes which were rinsed three times prior to filling. Standardizations were done at the beginning and end of each group of samples with a set of mid-concentration range standards prepared fresh for each run. Samples not analyzed immediately after collection were refrigerated and run the

* The first two digits represent the year and the last digits the month of the cruise.

following day. Sets of six different concentration standards were analyzed periodically to determine the deviation from linearity as a function of concentration, for the silicate, nitrate and phosphate analyses.  Final sample concentrations were corrected for deviations from linearity using a second order polynomial.

Samples for chlorophyll-a and phaeopigments were collected in calibrated 138 ml polyethylene bottles and filtered onto Whatman GF/F filters. The pigments were extracted in cold 90% acetone (Venrick and Hayward, 1984) for a mimimum of 24 hours. Chlorophyll a and pheopigment concentrations were determined from fluorescence readings before and after acidification with a Turner Designs Fluorometer Model 10-AU-005-CE (Yentsch and Menzel, 1963; Holm-Hansen et al., 1965).

Evaluation of the water sample data involved comparisons with the CTD data, adjacent stations and consideration of the variation of a property as a function of density or depth and the relationships with other properties (Klein, 1973). Precision estimates for routine analyses were made on CalCOFI cruise 9003 and are reported in SIO Ref. 91-4.

Primary Productivity Sampling

Primary productivity samples were taken each day shortly before local apparent noon (LAN). Primary production was estimated from ¹⁴C uptake using a simulated in situ technique. Light penetration was estimated from the Secchi depth (assuming that the 1% light level is three times the Secchi depth). The depths with ambient light intensities corresponding to light levels simulated by the on-deck incubators were identified and sampled on the rosette up-cast. Occasionally an extra bottle or two were tripped in addition to the usual 20 levels sampled in the combined rosette-productivity cast in order to maintain the normal sampling depth resolution. Triplicate samples (two light and one dark control) were drawn from each productivity sample depth into 250 ml polycarbonate incubation bottles. Samples were inoculated with 10 µCi of ¹⁴C as NaHCO₃ (200 µl of 50 µCi/ml stock) prepared in a 0.3 g/liter solution of sodium carbonate (Fitzwater et al., 1982). Samples were incubated from LAN to civil twilight in seawater-cooled incubators with neutral-density screens which simulate in situ light levels. At the end of the incubation, the samples were filtered onto Millipore HA filters and placed in scintillation vials. One half ml of 10% HCl was added to each sample. The sample was then allowed to sit, without a cap, at room temperature for 12 hours (after Lean and Burnison, 1979). Following this, 10 ml of scintillation cocktail were added to each sample and the samples were returned to SIO where the radioactivity was determined with a scintillation counter. Salinity, oxygen, nutrients, chlorophyll-a and phaeopigments were determined from all rosette productivity bottles.

Macrozooplankton Net Tows

Macrozooplankton was sampled with a 71 cm mouth diameter paired net (bongo net) equipped with 0.505mm plankton mesh. Bottom depth permitting, the nets were towed obliquely from 210 meters to the surface. The tow time for a standard tow was 21.5 minutes. Volumes filtered were determined from flowmeter readings and the mouth area of the net. Only one sample of each pair was retained and preserved. The biomass, as wet displacement volume, after removal of large (>5 ml) organisms, was determined in the laboratory ashore. These procedures are summarized in greater detail in Kramer et al. (1972). An Optical Plankton Counter (OPC, Dave Checkley, SIO) was routinely used in one side of the paired bongo net frame. The purpose of the OPC is to obtain information on the vertical distributions of size categories of zooplankton, using data from the counter, without affecting the ongoing time series of data obtained from the catches of the integrative bongo net.

Avifauna Observations (Point Reys Bird Observatory)

Sea birds were counted within a 300-meter wide strip off to one side of the ship. Counts were made while underway between stations during periods of daylight. These counts were summed over 20 nautical mile (nm) intervals, or the distance between consecutive stations, whichever was less. Included at the end of this report are individual maps of the most numerous bird species (individuals/nm).

Ancillary Programs

Several ancillary programs produced data on these cruises that are not presented in this report. These programs include:

1) Underway Data. Continuous near surface measurements of temperature, salinity and in vivo chlorophyll fluorescence were recorded from seawater pumped through the ship’s uncontaminated seawater system. Water was drawn from a depth of approximately 3 meters. The data were logged in one-minute averages using a Sea-Bird Electronics, Inc., SBE 45 MicroTSG Thermosalinograph and a Wetlabs Wetstar fluorometer.

2) ADCP. Continuous profiles of ocean currents and acoustic backscatter between 20 and 500 meters deep were measured along the shiptrack from a hull-mounted 150 kHz Acoustic Doppler Current Profiler (ADCP). The ADCP data were averaged over 3-minute intervals. Sixty 8-meter depth bins were recorded. (T. Chereskin, SIO)

3) Taxon-specific pigments. Water samples were collected from a depth of 10 m for the analysis of taxon-specific pigments (chlorophylls and carotenoids) by high-pressure liquid chromatography (R. Goericke, SIO).

4) Trace metals. Seawater samples from the surface and at depth were obtained for iron analysis (dissolved and total iron) at 33 stations using a trace metal-clean pole sampler and trace metal-clean GO-flo bottles. Iron addition incubations were also performed at 15 stations to assay for iron limitation in the phytoplankton community. (K. Barbeau, SIO).

5)  Temperature-dependent development of sardine and anchovy eggs. Sardine and anchovy eggs were colelcted using 303 um bongo nets. Three tows were conducted. Eggs were incubated at 10 different temperatures. Development stage was recorded using microscope visualization, and observed several times per day until hatching. The objective was to determine whether sardine and anchovy eggs have differential rates of development under different temperature regimes. (S. Glaser, SIO).
 
6) Microbial Diversity.  Water was collected from the surface and five meters depth at select stations to analyze temporal spatial patterns in bacterial community composition via the molecular fingerprinting technique ARISA (automated rRNA intergenic spacer assay). DNA from these samples was also used to investigate the abundance of aerobic anoxygenic phototrophic bacteria via quantitative PCR (QPCR).  Finally, ~100L of near surface seawater was collected from station 87.110 and used in a mesocosm experiment which investigated the impact of light removal on bacterial community composition. (Mike Schwalbach, Univ.So.Cal.)

7) Bio-optics. Apparent inherent optical properties of the top 100 meters of the water column were measured daily with a multi-spectral free fall radiometer. Backscattering properties of the top 300 meters of seawater
were also measured daily with a 6-channel backscattering meter. Water samples obtained from the CTD/rosette cast were analyzed for determination of absorption by particulate, detrital and soluble materials, HPLC determination of algal pigments. Water samples were also collected and analyzed for particulate organic carbon and particulate size distribution. Short-term photosynthesis-irradiance (P vs E) response was also determined for samples incubated with 14C sodium bicarbonate. Datasets of spectral solar irradiance, water leaving radiance and aerosol optical thickness were acquired during daylight hours en route and on stations using hand held SIMBADA radiometer, TriOS hyperspectral radiometer from JAXA of Japan, Portable Radiation Package (PRP) radiometer from NASA, for the calibration of the post Japanese satellite ocean color sensor Global Imager (GLI). (G. Mitchell, SIO)

8) Organic carbon. At each station several samples were drawn from the CTD for total organic carbon concentration profiles. Casts of 24 ten liter bottles to 1000 meters were filtered for stable isotope measurements of organic carbon.  Several solid phase extracts from filtered seawater were taken for chemical and isotope analyses of dissolved organic carbon (L. Aluwihare, SIO)

TABULATED DATA

CTD/Rosette Cast Data

The time reported is the Coordinated Universal Time (UTC) of the first rosette bottle trip on the up cast. The rosette bottles tripped on the up cast are reported as cast 2, where cast 1 is considered to be the down CTD profile. The sample number reported is the cast number followed by a two-digit rosette bottle number. Bottom depths, determined acoustically, have been corrected using British Admiralty Tables (Carter, 1980) and are reported in meters. Weather conditions have been coded using WMO code 4501. Secchi depths are reported for most daylight stations.

Data values from discreet sampled CTD rosette were interpolated and are reported for standard depths. Interpolated or extrapolated standard level data are noted by the footnote “ISL” printed after the depth. Multiple bottles tripped at the same depth to provide water for ancillary programs are not used in the calculation of standard depth data. Density-related parameters have been calculated from the International Equation of State of Seawater 1980 (UNESCO, 1981b). Computed values of potential temperature, sigma-theta, specific volume anomaly (SVA), and dynamic height or geopotential anomaly are included with both observed and interpolated standard depth levels.

On stations where primary productivity samples were drawn a footnote appears after each productivity depth sampled. The corresponding primary productivity data are reported in a separate section following the tabulated rosette cast data.

Primary Productivity Data

In addition to the normal hydrographic data that are reported in the rosette cast data section, the tabulated data include: the in situ light levels at which the samples were collected, the uptake from each of the replicate light bottles, uptake 1 and uptake 2 (which have been corrected for dark uptake by subtracting the dark value), the mean of the two uptake values and the dark uptake. The uptake values are totals for the incubation period. Also shown are the times of LAN, civil twilight, and the value of the mean uptake integrated from the surface to the deepest sample, assuming the shallowest value continues to the surface and that negative values (when dark uptake exceeds light uptake) are zero. The uptake data are reported to two significant digits (values <1.00) or one decimal (values >1.00). Precision of the higher production values may not warrant all of the digits presented. Incubation time, LAN, and civil twilight are given in local Pacific Standard Time (PST); to convert to UTC, add eight hours to the PST time. Incubation light intensities are listed in a footnote at the bottom of each page.

Macrozooplankton Data

Macrozooplankton biomass volumes are tabulated as total biomass volume (cm³/1000m³ strained) and as the total volume minus the volume of larger organisms under the heading “Small.” Tow times are given in local PST (+8) time.

FOOTNOTES

In addition to footnotes, special notations are used without footnotes because the meaning is always the same:

D: CTD salinity value listed in place of normal shipboard salinity analysis.

ISL: After a depth value indicates that this is an interpolated or extrapolated standard level.

U: Uncertain value. Values which are not used in interpolation because they seem to be in error without

apparent reason.